Isolation of single cells for protein therapeutics using microwell selection and Surface Plasmon Resonance imaging.
Anal Biochem
; 531: 45-47, 2017 08 15.
Article
in En
| MEDLINE
| ID: mdl-28545866
ABSTRACT
Here the feasibility is demonstrated that by combining Surface Plasmon Resonance Imaging (SPRi) and self-sorting microwell technology product secretion of individual cells can be monitored. Additionally isolation of the selected cells can be performed by punching the cells from the microwells using coordinates of the positions of microwells obtained with SPRi. Cells of interest can be retrieved sterile from the microwell array for further cultivation.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Separation
/
Surface Plasmon Resonance
/
Tissue Array Analysis
Limits:
Animals
/
Humans
Language:
En
Journal:
Anal Biochem
Year:
2017
Document type:
Article
Affiliation country:
Netherlands