Selective fluorescence detection method for selenide and selenol using monochlorobimane.
Anal Biochem
; 532: 1-8, 2017 09 01.
Article
in En
| MEDLINE
| ID: mdl-28552757
ABSTRACT
The low redox potential of selenide and selenol is physiologically important, as it confers efficient catalytic abilities to selenoproteins. Quantitative determination of selenol and selenide provide important clues for understanding the metabolism and physiological function of selenium. However, selective detection of selenol and selenide is extremely difficult because of their chemical similarity to thiol and sulfide. In this study, we established a highly sensitive, selective, quantitative, and simple method for detection of selenol and selenide, using a reaction with monochlorobimane (MCB), followed by ethyl acetate extraction of the product syn-(methyl,methyl)bimane. We analyzed selenide production from selenite, catalyzed by human glutathione reductase, and also determined selenide and selenol concentrations in Hepa1-6 cells using the MCB method, to demonstrate its practical applications. This study provides a new tool for selenium detection in biology.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pyrazoles
/
Sodium Selenite
/
Selenium Compounds
/
Carcinoma, Hepatocellular
/
Fluorescence
/
Liver Neoplasms
Type of study:
Diagnostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Anal Biochem
Year:
2017
Document type:
Article
Affiliation country:
Japan