A method for the isolation and characterization of functional murine monoclonal antibodies by single B cell cloning.

Carbonetti, Sara; Oliver, Brian G; Vigdorovich, Vladimir; Dambrauskas, Nicholas; Sack, Brandon; Bergl, Emilee; Kappe, Stefan H I; Sather, D Noah

Carbonetti, Sara; Oliver, Brian G; Vigdorovich, Vladimir; Dambrauskas, Nicholas; Sack, Brandon; Bergl, Emilee; Kappe, Stefan H I; Sather, D Noah.

Affiliation

Carbonetti S; Center for Infectious Disease Research (formerly Seattle BioMed), Seattle, WA, USA.
Oliver BG; Center for Infectious Disease Research (formerly Seattle BioMed), Seattle, WA, USA.
Vigdorovich V; Center for Infectious Disease Research (formerly Seattle BioMed), Seattle, WA, USA.
Dambrauskas N; Center for Infectious Disease Research (formerly Seattle BioMed), Seattle, WA, USA.
Sack B; Center for Infectious Disease Research (formerly Seattle BioMed), Seattle, WA, USA.
Bergl E; Center for Infectious Disease Research (formerly Seattle BioMed), Seattle, WA, USA.
Kappe SHI; Center for Infectious Disease Research (formerly Seattle BioMed), Seattle, WA, USA.
Sather DN; Center for Infectious Disease Research (formerly Seattle BioMed), Seattle, WA, USA. Electronic address: noah.sather@cidresearch.org.

J Immunol Methods ; 448: 66-73, 2017 09.

Article in En | MEDLINE | ID: mdl-28554543

Subject(s)

Antibodies, Monoclonal/immunology; Antibodies, Monoclonal/isolation & purification; Antigens/immunology; B-Lymphocytes/immunology; Clone Cells/immunology; Cloning, Molecular/methods; Malaria Vaccines/immunology; Malaria Vaccines/isolation & purification; Plasmodium falciparum/immunology; Protozoan Proteins/immunology; 5' Untranslated Regions; Animals; Antibodies, Monoclonal/biosynthesis; Antibodies, Monoclonal/genetics; Antibody Formation; Antibody Specificity; Antigens/administration & dosage; B-Lymphocytes/parasitology; CD40 Ligand/immunology; Cell Proliferation; Cell Separation/methods; Clone Cells/parasitology; Coculture Techniques; Flow Cytometry; HEK293 Cells; Humans; Immunization; Lymphocyte Activation; Malaria Vaccines/biosynthesis; Malaria Vaccines/genetics; Mice, Inbred BALB C; Polymerase Chain Reaction; Protozoan Proteins/administration & dosage; Workflow

Key words

Antibody; B cell; Cloning; FACS; Monoclonal; RACE; Recombinant

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plasmodium falciparum / B-Lymphocytes / Protozoan Proteins / Clone Cells / Cloning, Molecular / Malaria Vaccines / Antibodies, Monoclonal / Antigens Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: J Immunol Methods Year: 2017 Document type: Article Affiliation country: United States

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