[A Study on the establishment of immune thrombocytopenia model induced by anti-platelet GPâ
bα antibodies].
Zhonghua Xue Ye Xue Za Zhi
; 38(5): 390-393, 2017 May 14.
Article
in Zh
| MEDLINE
| ID: mdl-28565737
ABSTRACT
Objective:
To establish primary immune thrombocytopenia (ITP) animal model induced by anti-platelet membrane glycoprotein GPâ bα antibodies AN51 and R300.Methods:
Twenty guinea pigs (6-8 week) were divided into 4 groups. Five guinea pigs in each group were intravenously injected with different doses of AN51 (0.05, 0.1, 0.2 µg/g) and 0.2 µg/g IgG as control. The whole blood was collected from inner angular venous plexus. Platelets number was determined by an automated cell counter and Swiss-Jim method. Then, the similar protocol was used to establish ITP nude mice model by intraperitoneal injection of different concentrations of anti-platelet GPâ bα antibody R300, respectively.Results:
â Five minutes after intravenous injection of AN51 at 0.05, 0.1 and 0.2 µg/g, the platelet counts of guinea pigs reduced about 0-5%, 50%-60% and 70%-80% compared to the control group, respectively. The difference was statistically significant (P<0.01) . â¡Six hours after intraperitoneal injection of R300 at 0.05, 0.1, 0.2 µg/g, the platelet counts of nude mice decreased about 20%-30%, 60%-70% and 80%-90% compared to the control group, respectively. The difference was statistically significant (P<0.01) . The nude mice, injected 0.2 µg/g R300 once a day for 2 weeks, showed typical ITP clinical manifestations including large number of petechiaes or ecchymoses on limbs, head and abdomen.Conclusion:
AN51 at 0.2 µg/g and R300 at 0.2 µg/g could establish stable ITP model in guinea pigs and nude mice respectively.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Thrombocytopenia
/
Blood Platelets
Type of study:
Guideline
Limits:
Animals
/
Female
/
Humans
Language:
Zh
Journal:
Zhonghua Xue Ye Xue Za Zhi
Year:
2017
Document type:
Article
Affiliation country:
China