Novel in vivo experimental viability assays with high sensitivity and throughput capacity using a bdelloid rotifer.

Olah, Zita; Bush, Ashley I; Aleksza, David; Galik, Bence; Ivitz, Eszter; Macsai, Lilla; Janka, Zoltan; Karman, Zoltan; Kalman, Janos; Datki, Zsolt

Olah, Zita; Bush, Ashley I; Aleksza, David; Galik, Bence; Ivitz, Eszter; Macsai, Lilla; Janka, Zoltan; Karman, Zoltan; Kalman, Janos; Datki, Zsolt.

Affiliation

Olah Z; Department of Psychiatry, University of Szeged, Szeged, Hungary.
Bush AI; Oxidation Biology Unit, Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, Victoria, Australia.
Aleksza D; Institute of Plant Biology, Biological Research Centre, Szeged, Hungary.
Galik B; Bioinformatics & Scientific Computing, Vienna Biocenter Core Facilities, Vienna, Austria.
Ivitz E; Department of Psychiatry, University of Szeged, Szeged, Hungary.
Macsai L; Department of Psychiatry, University of Szeged, Szeged, Hungary.
Janka Z; Department of Psychiatry, University of Szeged, Szeged, Hungary.
Karman Z; Department of Psychiatry, University of Szeged, Szeged, Hungary.
Kalman J; Department of Psychiatry, University of Szeged, Szeged, Hungary.
Datki Z; Department of Psychiatry, University of Szeged, Szeged, Hungary. Electronic address: datkizsolt@gmail.com.

Ecotoxicol Environ Saf ; 144: 115-122, 2017 Oct.

Article in En | MEDLINE | ID: mdl-28605645

Subject(s)

Environmental Monitoring/methods; Hydrogen Peroxide/toxicity; Rotifera/drug effects; Sodium Azide/toxicity; Animals; Biological Assay; High-Throughput Screening Assays; Humans; Phylogeny; RNA, Ribosomal, 18S/genetics; Rotifera/genetics; Sensitivity and Specificity; Species Specificity; Survival Analysis

Key words

18S rDNA; Agarose (PubChem CID: 11966311); Bdelloid; Borate (PubChem CID: 26574); Culturing; EDTA (PubChem CID: 6049); Ethanol (PubChem CID: 702); High throughput assay; Hydrogen peroxide (PubChem CID: 784); Mastax; Oil-drop method; Oxidative stress; Pharmacological model; Philodina acuticornis odiosa; Rotifer; Sodium azide (PubChem CID: 33557); Sodium chloride (PubChem CID: 5234); TRIS (PubChem CID: 13286); XTT (PubChem CID: 497813)

Fulltext

Add to My VHL

XML

PubMed Links

Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rotifera / Environmental Monitoring / Sodium Azide / Hydrogen Peroxide Type of study: Diagnostic_studies Limits: Animals / Humans Language: En Journal: Ecotoxicol Environ Saf Year: 2017 Document type: Article Affiliation country: Hungary

Fulltext

Add to My VHL

XML

PubMed Links

Search on Google