Accurate virus quantitation using a Scanning Transmission Electron Microscopy (STEM) detector in a scanning electron microscope.
J Virol Methods
; 248: 136-144, 2017 10.
Article
in En
| MEDLINE
| ID: mdl-28668710
ABSTRACT
A method for accurate quantitation of virus particles has long been sought, but a perfect method still eludes the scientific community. Electron Microscopy (EM) quantitation is a valuable technique because it provides direct morphology information and counts of all viral particles, whether or not they are infectious. In the past, EM negative stain quantitation methods have been cited as inaccurate, non-reproducible, and with detection limits that were too high to be useful. To improve accuracy and reproducibility, we have developed a method termed Scanning Transmission Electron Microscopy - Virus Quantitation (STEM-VQ), which simplifies sample preparation and uses a high throughput STEM detector in a Scanning Electron Microscope (SEM) coupled with commercially available software. In this paper, we demonstrate STEM-VQ with an alphavirus stock preparation to present the method's accuracy and reproducibility, including a comparison of STEM-VQ to viral plaque assay and the ViroCyt Virus Counter.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Viruses
/
Microscopy, Electron, Scanning
/
Microscopy, Electron, Scanning Transmission
/
Viral Load
Language:
En
Journal:
J Virol Methods
Year:
2017
Document type:
Article
Affiliation country:
United States