Next Generation DNA Sequencing of Tissues from Infected Diabetic Foot Ulcers.
EBioMedicine
; 21: 142-149, 2017 Jul.
Article
in En
| MEDLINE
| ID: mdl-28669650
ABSTRACT
We used next generation DNA sequencing to profile the microbiome of infected Diabetic Foot Ulcers (DFUs). The microbiota was correlated to clinical parameters and treatment outcomes to determine if directed antimicrobial therapy based on conventional microbiological cultures are relevant based on genomic analysis. Patients≥18years presenting with a new Diabetic Foot Infection (DFI) who had not received topical or oral antimicrobials in the two weeks prior to presentation, were eligible for enrolment. Tissue punch biopsies were obtained from infected DFUs for analysis. Demographics, clinical and laboratory data were collected and correlated against microbiota data. Thirty-nine patients with infected DFUs were recruited over twelve-months. Shorter duration DFUs (Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Diabetic Foot
/
Metagenome
/
Metagenomics
/
Microbiota
Type of study:
Diagnostic_studies
Limits:
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
EBioMedicine
Year:
2017
Document type:
Article