Enhanced soluble production of cholera toxin B subunit in Escherichia coli by co-expression of SKP chaperones.
Protein Expr Purif
; 138: 1-6, 2017 Oct.
Article
in En
| MEDLINE
| ID: mdl-28673733
ABSTRACT
The cholera toxin B subunit (CTB) is a nontoxic portion of the cholera toxin that retains mucosal adjuvant properties. Expression of CTB in Escherichia coli is difficult as CTB aggregates and accumulates as insoluble inclusion bodies. To remedy this problem, the periplasmic chaperone, SKP, was investigated as possible co-expression partner to increase the solubility of recombinant CTB (rCTB) in E. coli. The result showed co-expression of SKP enhanced the soluble expression of rCTB in E. coli. Moreover, soluble rCTB was successfully expressed and secreted into the periplasmic space through the direction of the LTB leader signal. rCTB in periplasm was purified using an immobilized d-galactose resin; GM1-ELISA experiments showed that rCTB retains strong GM1 ganglioside-binding activity. Intranasal administration of ovalbumin (OVA) with rCTB significantly induced both mucosal and humoral immune responses specific to OVA. These data indicate that co-expression of the molecular chaperone SKP with CTB increased the solubility of rCTB while maintaining its function.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Adjuvants, Immunologic
/
Cholera Toxin
/
Immunity, Mucosal
/
S-Phase Kinase-Associated Proteins
/
Escherichia coli
Limits:
Animals
Language:
En
Journal:
Protein Expr Purif
Journal subject:
BIOLOGIA MOLECULAR
Year:
2017
Document type:
Article
Affiliation country:
China