Frequent generation of mutants with coincidental changes in multiple traits via ion-beam irradiation in soybean.
Genes Genet Syst
; 92(3): 153-161, 2018 Jan 20.
Article
in En
| MEDLINE
| ID: mdl-28674272
ABSTRACT
Ion beams are powerful mutagens that can induce novel mutants in plants. We previously established a system for producing a mutant population of soybean via ion-beam irradiation, isolated plants that had chlorophyll deficiency, and maintained their progeny via self-fertilization. Here we report the characterization of the progeny plants in terms of chlorophyll content, flowering time and isoflavone content in seeds. Chlorophyll deficiency in the leaf tissues was linked with reduced levels of isoflavones, the major flavonoid compounds accumulated in soybean seeds, which suggested the involvement of metabolic changes associated with the chlorophyll deficiency. Intriguingly, flowering time was frequently altered in plants that had a reduced level of chlorophyll in the leaf tissues. Plant lines that flowered either earlier or later than the wild-type plants were detected. The observed coincidental changes were presumed to be attributable to the following origins structural changes of DNA segments leading to the loss of multiple gene functions, or indirect effects of mutations that affect one of these traits, which were manifested as phenotypic changes in the background of the duplicated composition of the soybean genome.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Radiation, Ionizing
/
Glycine max
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Mutagenesis
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Genome, Plant
/
Quantitative Trait, Heritable
/
Mutation
Language:
En
Journal:
Genes Genet Syst
Journal subject:
BIOLOGIA MOLECULAR
/
BIOTECNOLOGIA
Year:
2018
Document type:
Article