Efficient Depletion of Essential Gene Products for Loss-of-Function Studies in Embryonic Stem Cells.

Berlivet, Soizik; Hmitou, Isabelle; Picaud, Hélène; Gérard, Matthieu

Berlivet, Soizik; Hmitou, Isabelle; Picaud, Hélène; Gérard, Matthieu.

Affiliation

Berlivet S; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette, France.
Hmitou I; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette, France.
Picaud H; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette, France.
Gérard M; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette, France. matthieu.gerard@cea.fr.

Methods Mol Biol ; 1622: 91-100, 2017.

Article in En | MEDLINE | ID: mdl-28674803

Subject(s)

Embryonic Stem Cells/metabolism; Genes, Essential; Loss of Function Mutation; Animals; Electroporation; Mice; Plasmids/genetics; RNA Interference; RNA, Small Interfering/genetics; Transfection

Key words

Electroporation; Embryonic stem cell; Essential genes; Puromycin selection; RNA interference; pHYPER; shRNA

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Genes, Essential / Embryonic Stem Cells / Loss of Function Mutation Limits: Animals Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2017 Document type: Article Affiliation country: France Country of publication: United States

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