Structure of the WD40-domain of human ATG16L1.
Protein Sci
; 26(9): 1828-1837, 2017 Sep.
Article
in En
| MEDLINE
| ID: mdl-28685931
ABSTRACT
Autophagy-related protein ATG16L1 is a component of the mammalian ATG12â¼ATG5/ATG16L1 complex, which acts as E3-ligase to catalyze lipidation of LC3 during autophagosome biogenesis. The N-terminal part of ATG16L1 comprises the ATG5-binding site and coiled-coil dimerization domain, both also present in yeast ATG16 and essential for bulk and starvation induced autophagy. While absent in yeast ATG16, mammalian ATG16L1 further contains a predicted C-terminal WD40-domain, which has been shown to be involved in mediating interaction with diverse factors in the context of alternative functions of autophagy, such as inflammatory control and xenophagy. In this work, we provide detailed information on the domain boundaries of the WD40-domain of human ATG16L1 and present its crystal structure at a resolution of 1.55 Å.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Autophagy-Related Proteins
/
WD40 Repeats
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Protein Sci
Journal subject:
BIOQUIMICA
Year:
2017
Document type:
Article
Affiliation country:
Germany