Integrated process development-a robust, rapid method for inclusion body harvesting and processing at the microscale level.

Walther, Cornelia; Kellner, Martin; Berkemeyer, Matthias; Brocard, Cécile; Dürauer, Astrid

Walther, Cornelia; Kellner, Martin; Berkemeyer, Matthias; Brocard, Cécile; Dürauer, Astrid.

Affiliation

Walther C; a Department of Biotechnology , University of Natural Resources and Life Sciences Vienna , Vienna , Austria.
Kellner M; b Biopharma Process Science Boehringer-Ingelheim Regional Center Vienna , Vienna , Austria.
Berkemeyer M; b Biopharma Process Science Boehringer-Ingelheim Regional Center Vienna , Vienna , Austria.
Brocard C; b Biopharma Process Science Boehringer-Ingelheim Regional Center Vienna , Vienna , Austria.
Dürauer A; b Biopharma Process Science Boehringer-Ingelheim Regional Center Vienna , Vienna , Austria.

Prep Biochem Biotechnol ; 47(9): 874-880, 2017 Oct 21.

Article in En | MEDLINE | ID: mdl-28703683

Subject(s)

Escherichia coli/cytology; Inclusion Bodies/chemistry; Cell Fractionation/economics; Cell Fractionation/methods; Escherichia coli/chemistry; Escherichia coli/genetics; Inclusion Bodies/genetics; Miniaturization/methods; Recombinant Proteins/genetics; Recombinant Proteins/isolation & purification; Time Factors

Key words

Cell disruption; E. coli; high-throughput; miniaturization; protein refolding; scale-up

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Inclusion Bodies / Escherichia coli Type of study: Prognostic_studies Language: En Journal: Prep Biochem Biotechnol Journal subject: BIOQUIMICA / BIOTECNOLOGIA Year: 2017 Document type: Article Affiliation country: Austria Country of publication: United kingdom

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