Rapid real-time recirculating PCR using localized surface plasmon resonance (LSPR) and piezo-electric pumping.
Lab Chip
; 17(16): 2821-2830, 2017 08 08.
Article
in En
| MEDLINE
| ID: mdl-28703830
ABSTRACT
Rapid detection and characterization of pathogens in patients with bloodstream infections (BSIs) is a persistent problem for modern medicine, as current techniques are slow or provide incomplete diagnostic information. Real-time polymerase chain reaction (qPCR) allows specific detection of a wide range of targets and quantification of pathogenic burdens to aid in treatment planning. However, new technological advances are required for a rapid and multiplex implementation of qPCR in clinical applications. In this paper, the feasibility of a novel microfluidic platform for qPCR is presented, integrating highly sensitive, label-free localized surface plasmon resonance (LSPR) imaging of DNA hybridization into a recirculating chip design for real-time analysis. Single target and multiplex detection of DNA target amplification are demonstrated, with a limit of detection of 5 fg µL-1 of E. coli DNA for single target PCR, correlating with approximately 300 bacteria per mL. The results of this study demonstrate the potential of this platform for simultaneous real-time detection of multiple target genes within 15 minutes that could provide live saving benefits in patients with BSIs.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Surface Plasmon Resonance
/
Microfluidic Analytical Techniques
/
Real-Time Polymerase Chain Reaction
Language:
En
Journal:
Lab Chip
Journal subject:
BIOTECNOLOGIA
/
QUIMICA
Year:
2017
Document type:
Article
Affiliation country:
United States