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Genetic Transformation of Date Palm Via Microprojectile Bombardment.
Mousavi, Mousa; Mousavi, Amir; Habashi, Ali A; Arzani, Kazem; Dehsara, Bahareh; Brajeh, Mohsen.
Affiliation
  • Mousavi M; Department of Horticulture Science, Faculty of Agriculture, Shahid Chamran University, Ahvaz (Ahwaz), Iran. m.mousavi@scu.ac.ir.
  • Mousavi A; National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
  • Habashi AA; Department of Tissue Culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran.
  • Arzani K; Department of Horticulture Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
  • Dehsara B; National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
  • Brajeh M; Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahid Chamran University, Ahvaz (Ahwaz), Iran.
Methods Mol Biol ; 1637: 269-280, 2017.
Article in En | MEDLINE | ID: mdl-28755352
ABSTRACT
Efficient protocols for date palm embryogenic callus and somatic embryo transformation with uidA gene are described in this chapter. The embryogenic callus transformation procedure is 1.6 µm gold particle size coated with 2.5 µg DNA (pAct1-D plasmid), 1100 psi helium pressure, 9 cm target distance, 26 inHg vacuum pressure, 3 mm distance between the rupture disk and macrocarrier, and osmotic pretreatment with 0.4 M mannitol followed by 60 min air desiccation. The somatic embryo transformation procedure is 0.6 µm gold particle size coated with 2.5 µg DNA (pAct1-D plasmid), 1350 psi helium pressure, 6 cm target distance, 28 inHg vacuum pressure, 3 mm distance between the rupture disk and macrocarrier, and osmotic pretreatment with 0.4 M mannitol followed by 60 min air desiccation. Protocols for analysis of the transgenic plantlets have also been described.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plants, Genetically Modified / Biolistics / Phoeniceae Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2017 Document type: Article Affiliation country: Iran
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plants, Genetically Modified / Biolistics / Phoeniceae Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2017 Document type: Article Affiliation country: Iran