Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR.
Methods Mol Biol
; 1635: 345-358, 2017.
Article
in En
| MEDLINE
| ID: mdl-28755379
ABSTRACT
Conformational studies of membrane proteins remain a challenge in the field of structural biology, and in particular the investigation of the proteins in a native-like lipid environment. Solid-state NMR presents a valuable opportunity for this, and we present here three critical steps in the solid-state NMR sample preparation, i.e., membrane reconstitution of the protein in native lipids, rotor filling, and sample quality assessment, at the example of the Bacillus subtilis ATP-binding cassette transporter BmrA.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bacillus subtilis
/
Membrane Proteins
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2017
Document type:
Article
Affiliation country:
France