Profiling of cardiolipins and their hydroperoxides in HepG2 cells by LC/MS.
Anal Bioanal Chem
; 409(24): 5735-5745, 2017 Sep.
Article
in En
| MEDLINE
| ID: mdl-28762068
ABSTRACT
Cardiolipin (CL) exists as crucial functional phospholipid in mitochondria. The oxidation of CL is concerned with mitochondrial dysfunction and various diseases. As main oxidation products, CL hydroperoxide (CL-OOH) plays a key role in intermediating oxidative reaction. Thus, direct analysis of CL-OOH is of great interest. In the present study, CL and CL-OOH profiles were analyzed in oxidized HepG2 cell lipid via HPLC-Orbitrap MS/MS. Furthermore, the contents of individual molecular species were compared between intact and AAPH-oxidized HepG2 cells. In total, 46 CL and 18 CL-OOH were identified from oxidized cell lipids, while 21 CL and 9 CL-OOH were detected in AAPH-treated cells. Most CL depleted significantly after AAPH inducement, with percentages varying from 8.3% (CL707) to 73.7% (CL724), depending on fatty acyl composition. While almost all the CL-OOH remarkably increased, among them 686-, 726-, and 727-OOHs were only detected in AAPH-treated cells. CL685- and CL684-OOH were the most abundant species, while CL705-OOH among all the species expressed the highest oxidation percentage of the corresponding CL. Our results showed practical separation, identification, and semi-quantitation of CL-OOH species, which could contribute to approaches to lipidomic analysis of CL and CL-OOH, as well as tracing biomarkers in mitochondrial oxidative stress diagnosis. Graphical abstract Illustration represents cardiolipin hydroperoxide structure and its content increasing in AAPH-treated HepG2 cells by LC/MS analysis.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peroxides
/
Cardiolipins
/
Hepatocytes
Limits:
Humans
Language:
En
Journal:
Anal Bioanal Chem
Year:
2017
Document type:
Article
Affiliation country:
Japan