Bone marrow cell migration to the heart in a chimeric mouse model of acute chagasic disease.
Mem Inst Oswaldo Cruz
; 112(8): 551-560, 2017 Aug.
Article
in En
| MEDLINE
| ID: mdl-28767980
ABSTRACT
BACKGROUND:
Chagas disease is a public health problem caused by infection with the protozoan Trypanosoma cruzi. There is currently no effective therapy for Chagas disease. Although there is some evidence for the beneficial effect of bone marrow-derived cells in chagasic disease, the mechanisms underlying their effects in the heart are unknown. Reports have suggested that bone marrow cells are recruited to the chagasic heart; however, studies using chimeric mouse models of chagasic cardiomyopathy are rare.OBJECTIVES:
The aim of this study was to investigate the migration of bone marrow cells to the heart after T. cruzi infection in a model of chagasic disease in chimeric mice.METHODS:
To obtain chimerical mice, wild-type (WT) C57BL6 mice were exposed to full body irradiation (7 Gy), causing bone marrow ablation. Then, bone marrow cells from green fluorescent protein (GFP)-transgenic mice were infused into the mice. Graft effectiveness was confirmed by flow cytometry. Experimental mice were divided into four groups (i) infected chimeric (iChim) mice; (ii) infected WT (iWT) mice, both of which received 3 × 104 trypomastigotes of the Brazil strain; (iii) non-infected chimeric (Chim) mice; and (iv) non-infected WT mice.FINDINGS:
At one-month post-infection, iChim and iWT mice showed first degree atrioventricular block with decreased heart rate and treadmill exercise parameters compared to those in the non-infected groups. MAINCONCLUSIONS:
iChim mice showed an increase in parasitaemia, myocarditis, and the presence of amastigote nests in the heart tissue compared to iWT mice. Flow cytometry analysis did not detect haematopoietic progenitor cells in the hearts of infected mice. Furthermore, GFP+ cardiomyocytes were not detected in the tissues of chimeric mice.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bone Marrow Cells
/
Cell Movement
/
Chagas Disease
/
Myocardium
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Mem Inst Oswaldo Cruz
Year:
2017
Document type:
Article
Affiliation country:
Brazil