ACBD3 functions as a scaffold to organize the Golgi stacking proteins and a Rab33b-GAP.
FEBS Lett
; 591(18): 2793-2802, 2017 09.
Article
in En
| MEDLINE
| ID: mdl-28777890
ABSTRACT
Golgin45 plays important roles in Golgi stack assembly and is known to bind both the Golgi stacking protein GRASP55 and Rab2 in the medial-Golgi cisternae. In this study, we sought to further characterize the cisternal adhesion complex using a proteomics approach. We report here that Acyl-CoA binding domain containing 3 (ACBD3) is likely to be a novel binding partner of Golgin45. ACBD3 interacts with Golgin45 via its GOLD domain, while its co-expression significantly increases Golgin45 targeting to the Golgi. Furthermore, ACBD3 recruits TBC1D22, a Rab33b GTPase activating protein (GAP), to a large multi-protein complex containing Golgin45 and GRASP55. These results suggest that ACBD3 may provide a scaffolding to organize the Golgi stacking proteins and a Rab33b-GAP at the medial-Golgi.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Rab GTP-Binding Proteins
/
GTPase-Activating Proteins
/
Adaptor Proteins, Signal Transducing
/
Golgi Apparatus
/
Membrane Proteins
Limits:
Humans
Language:
En
Journal:
FEBS Lett
Year:
2017
Document type:
Article
Affiliation country:
China