Liquefied Wood as Inexpensive Precursor-Feedstock for Bio-Mediated Incorporation of (R)-3-Hydroxyvalerate into Polyhydroxyalkanoates.
Materials (Basel)
; 8(9): 6543-6557, 2015 Sep 23.
Article
in En
| MEDLINE
| ID: mdl-28793581
ABSTRACT
Liquefied wood (LW) prepared in a microwave process was applied as a novel; inexpensive precursor feedstock for incorporation of (R)-3-hydroxyvalerate (3HV) into polyhydroxyalkanoate (PHA) biopolyesters in order to improve the biopolyester's material quality; Cupriavidus necator was applied as microbial production strain. For proof of concept, pre-experiments were carried out on a shake flask scale using different mixtures of glucose and LW as carbon source. The results indicate that LW definitely acts as a 3HV precursor, but, at the same time, displays toxic effects on C. necator at concentrations exceeding 10 g/L. Based on these findings, PHA biosynthesis under controlled conditions was performed using a fed-batch feeding regime on a bioreactor scale. As major outcome, a poly(3HB-co-0.8%-3HV) copolyester was obtained displaying a desired high molar mass of Mw = 5.39 × 105 g/mol at low molar-mass dispersity (DM of 1.53), a degree of crystallinity (Xc) of 62.1%, and melting temperature Tm (176.3 °C) slightly lower than values reported for poly([R]-3-hydroxybutyrate) (PHB) homopolyester produced by C. necator; thus, the produced biopolyester is expected to be more suitable for polymer processing purposes.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Materials (Basel)
Year:
2015
Document type:
Article
Affiliation country:
Austria