Kinetic analyses of phosphorylated and non-phosphorylated eIFiso4E binding to mRNA cap analogues.

Khan, Mateen A; Goss, Dixie J

Khan, Mateen A; Goss, Dixie J.

Affiliation

Khan MA; Department of Chemistry and Biochemistry, Hunter College of the City University of New York, New York, NY 10065, USA; Department of Life Sciences, College of Science and General Studies, Alfaisal University, Riyadh 11533, KSA, Saudi Arabia. Electronic address: matkhan@alfaisal.edu.
Goss DJ; Department of Chemistry and Biochemistry, Hunter College of the City University of New York, New York, NY 10065, USA. Electronic address: dgoss@hunter.cuny.edu.

Int J Biol Macromol ; 106: 387-395, 2018 Jan.

Article in En | MEDLINE | ID: mdl-28797816

Subject(s)

Eukaryotic Initiation Factor-4E/metabolism; Peptide Chain Initiation, Translational; Plant Proteins/biosynthesis; RNA Cap Analogs/metabolism; RNA Caps/metabolism; Triticum/metabolism; Cloning, Molecular; Escherichia coli/genetics; Escherichia coli/metabolism; Eukaryotic Initiation Factor-4E/genetics; Gene Expression; Humans; Kinetics; Phosphorylation; Plant Proteins/genetics; Protein Binding; RNA Caps/genetics; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Thermodynamics; Triticum/genetics

Key words

Fluorescence spectroscopy; Phosphorylation; Stopped-Flow kinetics

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptide Chain Initiation, Translational / Plant Proteins / Triticum / RNA Cap Analogs / RNA Caps / Eukaryotic Initiation Factor-4E Limits: Humans Language: En Journal: Int J Biol Macromol Year: 2018 Document type: Article Country of publication: Netherlands

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