Auxin-Induced Modulation of ETTIN Activity Orchestrates Gene Expression in Arabidopsis.
Plant Cell
; 29(8): 1864-1882, 2017 Aug.
Article
in En
| MEDLINE
| ID: mdl-28804059
The phytohormone auxin governs crucial developmental decisions throughout the plant life cycle. Auxin signaling is effectuated by auxin response factors (ARFs) whose activity is repressed by Aux/IAA proteins under low auxin levels, but relieved from repression when cellular auxin concentrations increase. ARF3/ETTIN (ETT) is a conserved noncanonical Arabidopsis thaliana ARF that adopts an alternative auxin-sensing mode of translating auxin levels into multiple transcriptional outcomes. However, a mechanistic model for how this auxin-dependent modulation of ETT activity regulates gene expression has not yet been elucidated. Here, we take a genome-wide approach to show how ETT controls developmental processes in the Arabidopsis shoot through its auxin-sensing property. Moreover, analysis of direct ETT targets suggests that ETT functions as a central node in coordinating auxin dynamics and plant development and reveals tight feedback regulation at both the transcriptional and protein-interaction levels. Finally, we present an example to demonstrate how auxin sensitivity of ETT-protein interactions can shape the composition of downstream transcriptomes to ensure specific developmental outcomes. These results show that direct effects of auxin on protein factors, such as ETT-TF complexes, comprise an important part of auxin biology and likely contribute to the vast number of biological processes affected by this simple molecule.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nuclear Proteins
/
Arabidopsis
/
Gene Expression Regulation, Plant
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Arabidopsis Proteins
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DNA-Binding Proteins
/
Indoleacetic Acids
Type of study:
Prognostic_studies
Language:
En
Journal:
Plant Cell
Journal subject:
BOTANICA
Year:
2017
Document type:
Article
Affiliation country:
United kingdom
Country of publication:
United kingdom