Measurements of ionic concentrations along with endocochlear potential in wild-type and claudin 14 knockout mice.
Auris Nasus Larynx
; 45(3): 421-426, 2018 Jun.
Article
in En
| MEDLINE
| ID: mdl-28811056
ABSTRACT
OBJECTIVE:
To examine whether the changes in endolymphatic ion concentrations were involved in hair cells degeneration in claudin-14 knockout (KO) mice (Cldn14-/-), we measured the endocochlear potential (EP) along with concentrations of K+, Na+, H+, or Ca2+ ([K]e, [Na]e, pHe, [Ca]e) in Cldn14-/-, in which hair cells were selectively damaged, and compared with measurements in wild type mice (Wt).METHODS:
We used the Cldn14-/- from 3 weeks of age, in which the auditory brain responses (ABR) was severely diminished. Using double-barreled ion-selective microelectrodes, we measured [K]e, [Na]e, pHe, and [Ca]e in both Wt and Cldn14-/- at 8-10 weeks of age.RESULTS:
(1) In Wt, the EP was +92mV. [K]e, [Na]e, pHe, and [Ca]e were 169mM, â¼1.0mM, 7.50, and 395nM, respectively. In the Cldn14-/-, the EP was +96mV. [K]e, [Na]e, pHe, and [Ca]e were 167mM, â¼1.0mM, 7.73, and 179nM, respectively. No significant differences in the above values were observed between Wt and Cldn14-/-. (2) A significant linear correlation between EP and [Ca]e (R=0.93) was observed for both Wt and Cldn14-/-, but no correlation was observed between EP and K+, Na+, or H+.CONCLUSION:
These findings suggest that (1) the changes in endolymphatic ion concentrations might not be involved in hair cells degeneration in Cldn14-/-, (2) [Ca]e might be regulated by EP in both Wt and Cldn14-/-.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cochlea
/
Endolymph
/
Claudins
/
Hair Cells, Auditory
/
Membrane Potentials
Limits:
Animals
Language:
En
Journal:
Auris Nasus Larynx
Year:
2018
Document type:
Article
Affiliation country:
Japan
Publication country:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS