DNMT3A-mediated down-regulation of microRNA-105 promotes gastric cancer cell proliferation.
Eur Rev Med Pharmacol Sci
; 21(15): 3377-3383, 2017 Aug.
Article
in En
| MEDLINE
| ID: mdl-28829505
ABSTRACT
OBJECTIVE:
It has been well-established that microRNAs (miRNAs), a class of short non-coding RNA molecules, play an important role in the development of gastric cancer. In the present study, we focused on miR-105, a novel miRNA not previously linked to gastric cancer. PATIENTS ANDMETHODS:
36 paired surgically resected gastric cancer tissues and matched adjacent normal tissues were used to detect the expression of miR-105. AGS cells were used to overexpress or silence of miR-105 and to determine its effect on several tumorigenic properties. A cell proliferation enzyme-linked immunosorbent assay was used to analyze the incorporation of BrdU during DNA synthesis of AGS cells. Total cDNA from AGS cells was used to amplify the 3'-UTR of YY1 by PCR and luciferase activity was determined using the Dual-Luciferase Reporter Assay SystemRESULTS:
We found that expression of miR-105 was reduced in gastric cancer tissues, compared with adjacent normal tissues, due to hypermethylation at its promoter region. Overexpression of miR-105 suppressed, whereas its inhibition promoted cell viability and proliferation. We further identified Yin Yang 1 (YY1) as a direct target of miR-105, by which miR-105 exerted its anti-proliferative role. Moreover, we found that DNMT3A was responsible for the down-regulation of miR-105 in gastric cancer cells.CONCLUSIONS:
Our data demonstrate that miR-105 inhibits gastric cancer cell proliferation and progression, which might provide a therapeutical target for cancer therapy.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Stomach Neoplasms
/
MicroRNAs
/
DNA (Cytosine-5-)-Methyltransferases
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Eur Rev Med Pharmacol Sci
Journal subject:
FARMACOLOGIA
/
TOXICOLOGIA
Year:
2017
Document type:
Article
Affiliation country:
China