An enzymatic strategy to asymmetrically branched N-glycans.
Org Biomol Chem
; 15(35): 7258-7262, 2017 Sep 13.
Article
in En
| MEDLINE
| ID: mdl-28832049
ABSTRACT
An enzymatic strategy was developed to generate asymmetrically branched N-glycans from natural sources by using a panel of glycosidases and glycosyltransferases. Briefly, LacZ ß-galactosidase was employed to selectively trim symmetrically branched N-glycans isolated from bovine fetuin. The yielding structures were then converted to asymmetrically branched core structures by robust glycosyltransferase for further extension.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polysaccharides
/
Glycosyltransferases
/
Beta-Galactosidase
Limits:
Animals
Language:
En
Journal:
Org Biomol Chem
Journal subject:
BIOQUIMICA
/
QUIMICA
Year:
2017
Document type:
Article
Affiliation country:
United States