Preparation and characterization of alginate and gelatin microcapsules containing Lactobacillus rhamnosus.
An Acad Bras Cienc
; 89(3): 1601-1613, 2017.
Article
in En
| MEDLINE
| ID: mdl-28876396
ABSTRACT
This paper describes the preparation and characterization of alginate beads coated with gelatin and containing Lactobacillus rhamnosus. Capsules were obtained by extrusion method using CaCl2 as cross linker. An experimental design was performed using alginate and gelatin concentrations as the variables investigated, while the response variable was the concentration of viable cells. Beads were characterized in terms of size, morphology, scanning electron microscopy (SEM), moisture content, Fourier Transform Infrared Spectrometry (FTIR), thermal behavior and cell viability during storage. The results showed that the highest concentration of viable cells (4.2 x 109 CFU/g) was obtained for 1 % w/v of alginate and 0.1 % w/v of gelatin. Capsules were predominantly spherical with a rough surface, a narrow size distribution ranging from 1.53 to 1.90 mm and a moisture content of 97.70 ± 0.03 %. Furthermore, FTIR and thermogravimetric analysis indicated an interaction between alginate-gelatin. Cell concentration of alginate/gelatin microcapsules was 105 CFU/g after 4 months of storage at 8 oC.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Capsules
/
Probiotics
/
Drug Stability
/
Alginates
/
Lacticaseibacillus rhamnosus
/
Gelatin
Language:
En
Journal:
An Acad Bras Cienc
Year:
2017
Document type:
Article
Affiliation country:
Brazil