Cutting Edge: Endogenous IFN-ß Regulates Survival and Development of Transitional B Cells.
J Immunol
; 199(8): 2618-2623, 2017 10 15.
Article
in En
| MEDLINE
| ID: mdl-28904124
ABSTRACT
The transitional stage of B cell development is a formative stage in the spleen where autoreactive specificities are censored as B cells gain immune competence, but the intrinsic and extrinsic factors regulating survival of transitional stage 1 (T1) B cells are unknown. We report that B cell expression of IFN-ß is required for optimal survival and TLR7 responses of transitional B cells in the spleen and was overexpressed in T1 B cells from BXD2 lupus-prone mice. Single-cell gene expression analysis of B6 Ifnb+/+ versus B6 Ifnb-/- T1 B cells revealed heterogeneous expression of Ifnb in wild-type B cells and distinct gene expression patterns associated with endogenous IFN-ß. Single-cell analysis of BXD2 T1 B cells revealed that Ifnb is expressed in early T1 B cell development with subsequent upregulation of Tlr7 and Ifna1 Together, these data suggest that T1 B cell expression of IFN-ß plays a key role in regulating responsiveness to external factors.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spleen
/
Lupus Nephritis
/
B-Lymphocytes
/
Interferon-beta
/
Precursor Cells, B-Lymphoid
Limits:
Animals
Language:
En
Journal:
J Immunol
Year:
2017
Document type:
Article