Quantitative imaging of Candida utilis and its organelles by soft X-ray Nano-CT.
J Microsc
; 270(1): 64-70, 2018 04.
Article
in En
| MEDLINE
| ID: mdl-28960304
ABSTRACT
Soft X-ray microscopy has excellent characteristics for imaging cells and subcellular structures. In this paper, the yeast strain, Candida utilis, was imaged by soft X-ray microscopy and three-dimensional volumes were reconstructed with the SART-TV method. We performed segmentation on the reconstruction in three dimensions and identified several types of subcellular architecture within the specimen cells based on their linear absorption coefficient (LAC) values. Organelles can be identified by the correlation between the soft X-ray LAC values and the subcellular architectures. Quantitative analyses of the volume ratio of organelles to whole cell in different phases were also carried out according to the three-dimensional datasets. With such excellent features, soft X-ray imaging has a great influence in the field of biological cellular and subcellular research.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Candida
/
Organelles
/
Tomography, X-Ray Computed
/
Imaging, Three-Dimensional
/
Electron Probe Microanalysis
Type of study:
Prognostic_studies
Language:
En
Journal:
J Microsc
Year:
2018
Document type:
Article
Country of publication:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM