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Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation.
Edupuganti, Raghu Ram; Harikumar, Arigela; Aaronson, Yair; Biran, Alva; Sailaja, Badi Sri; Nissim-Rafinia, Malka; Azad, Gajendra Kumar; Cohen, Malkiel A; Park, Jung Eun; Shivalila, Chikdu S; Markoulaki, Styliani; Sze, Siu Kwan; Jaenisch, Rudolf; Meshorer, Eran.
Affiliation
  • Edupuganti RR; The Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 91904, Israel.
  • Harikumar A; The Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 91904, Israel.
  • Aaronson Y; The Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 91904, Israel.
  • Biran A; The Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 91904, Israel.
  • Sailaja BS; The Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 91904, Israel.
  • Nissim-Rafinia M; The Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 91904, Israel.
  • Azad GK; The Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 91904, Israel; The Edmond and Lily Safra Center for Brain Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
  • Cohen MA; Whitehead Institute for Biomedical Research, Cambridge, MA, USA.
  • Park JE; School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.
  • Shivalila CS; Whitehead Institute for Biomedical Research, Cambridge, MA, USA.
  • Markoulaki S; Whitehead Institute for Biomedical Research, Cambridge, MA, USA.
  • Sze SK; School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.
  • Jaenisch R; Whitehead Institute for Biomedical Research, Cambridge, MA, USA.
  • Meshorer E; The Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 91904, Israel; The Edmond and Lily Safra Center for Brain Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel. Electronic address: m
Stem Cell Reports ; 9(4): 1291-1303, 2017 10 10.
Article in En | MEDLINE | ID: mdl-28966118
Embryonic stem cells (ESCs) are regulated by pluripotency-related transcription factors in concert with chromatin regulators. To identify additional stem cell regulators, we screened a library of endogenously labeled fluorescent fusion proteins in mouse ESCs for fluorescence loss during differentiation. We identified SET, which displayed a rapid isoform shift during early differentiation from the predominant isoform in ESCs, SETα, to the primary isoform in differentiated cells, SETß, through alternative promoters. SETα is selectively bound and regulated by pluripotency factors. SET depletion causes proliferation slowdown and perturbed neuronal differentiation in vitro and developmental arrest in vivo, and photobleaching methods demonstrate SET's role in maintaining a dynamic chromatin state in ESCs. This work identifies an important regulator of pluripotency and early differentiation, which is controlled by alternative promoter usage.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Differentiation / Promoter Regions, Genetic / Gene Expression Regulation, Developmental / TATA-Binding Protein Associated Factors / Transcription Factor TFIID / Histone Acetyltransferases / Embryonic Stem Cells / Neoplasm Proteins / Nerve Tissue Proteins Type of study: Prognostic_studies Limits: Animals Language: En Journal: Stem Cell Reports Year: 2017 Document type: Article Affiliation country: Israel Country of publication: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Differentiation / Promoter Regions, Genetic / Gene Expression Regulation, Developmental / TATA-Binding Protein Associated Factors / Transcription Factor TFIID / Histone Acetyltransferases / Embryonic Stem Cells / Neoplasm Proteins / Nerve Tissue Proteins Type of study: Prognostic_studies Limits: Animals Language: En Journal: Stem Cell Reports Year: 2017 Document type: Article Affiliation country: Israel Country of publication: United States