Novel cell surface luciferase reporter for high-throughput yeast one-hybrid screens.
Nucleic Acids Res
; 45(18): e157, 2017 Oct 13.
Article
in En
| MEDLINE
| ID: mdl-28985361
ABSTRACT
Gene-centered yeast one-hybrid (Y1H) screens provide a powerful and effective strategy to identify transcription factor (TF)-promoter interactions. While genome-wide TF ORFeome clone collections are increasingly available, screening protocols have limitations inherent to the properties of the enzymatic reaction used to identify interactions and to the procedure required to perform the assay in a high-throughput format. Here, we present the development and validation of a streamlined strategy for quantitative and fully automated gene-centered Y1H screens using a novel cell surface Gaussia luciferase reporter.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Genes, Reporter
/
Two-Hybrid System Techniques
/
High-Throughput Screening Assays
/
Luciferases
Type of study:
Prognostic_studies
Language:
En
Journal:
Nucleic Acids Res
Year:
2017
Document type:
Article
Affiliation country:
United States
Publication country:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM