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Introducing site-specific cysteines into nanobodies for mercury labelling allows de novo phasing of their crystal structures.
Hansen, Simon Boje; Laursen, Nick Stub; Andersen, Gregers Rom; Andersen, Kasper R.
Affiliation
  • Hansen SB; Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10C, 8000 Aarhus, Denmark.
  • Laursen NS; Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10C, 8000 Aarhus, Denmark.
  • Andersen GR; Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10C, 8000 Aarhus, Denmark.
  • Andersen KR; Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10C, 8000 Aarhus, Denmark.
Acta Crystallogr D Struct Biol ; 73(Pt 10): 804-813, 2017 Oct 01.
Article in En | MEDLINE | ID: mdl-28994409
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cysteine / Single-Domain Antibodies / Mercury Limits: Animals / Humans Language: En Journal: Acta Crystallogr D Struct Biol Year: 2017 Document type: Article Affiliation country: Denmark
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cysteine / Single-Domain Antibodies / Mercury Limits: Animals / Humans Language: En Journal: Acta Crystallogr D Struct Biol Year: 2017 Document type: Article Affiliation country: Denmark