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In Vivo Production of Monoclonal Antibodies by Gene Transfer via Electroporation Protects against Lethal Influenza and Ebola Infections.
Andrews, Chasity D; Luo, Yang; Sun, Ming; Yu, Jian; Goff, Arthur J; Glass, Pamela J; Padte, Neal N; Huang, Yaoxing; Ho, David D.
Affiliation
  • Andrews CD; Aaron Diamond AIDS Research Center, The Rockefeller University, New York, NY 10016, USA.
  • Luo Y; Aaron Diamond AIDS Research Center, The Rockefeller University, New York, NY 10016, USA.
  • Sun M; Aaron Diamond AIDS Research Center, The Rockefeller University, New York, NY 10016, USA.
  • Yu J; Aaron Diamond AIDS Research Center, The Rockefeller University, New York, NY 10016, USA.
  • Goff AJ; US Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USA.
  • Glass PJ; US Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USA.
  • Padte NN; Aaron Diamond AIDS Research Center, The Rockefeller University, New York, NY 10016, USA.
  • Huang Y; Aaron Diamond AIDS Research Center, The Rockefeller University, New York, NY 10016, USA.
  • Ho DD; Aaron Diamond AIDS Research Center, The Rockefeller University, New York, NY 10016, USA.
Mol Ther Methods Clin Dev ; 7: 74-82, 2017 Dec 15.
Article in En | MEDLINE | ID: mdl-29034261
Monoclonal antibodies (mAbs) have wide clinical utility, but global access is limited by high costs and impracticalities associated with repeated passive administration. Here, we describe an optimized electroporation-based DNA gene transfer platform technology that can be utilized for production of functional mAbs in vivo, with the potential to reduce costs and administration burdens. We demonstrate that multiple mAbs can be simultaneously expressed at protective concentrations for a protracted period of time using DNA doses and electroporation conditions that are feasible clinically. The expressed mAbs could also protect mice against lethal influenza or Ebola virus challenges. Our findings suggest that this DNA gene transfer platform technology could be a game-changing advance that expands access to effective mAb therapeutics globally.
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Mol Ther Methods Clin Dev Year: 2017 Document type: Article Affiliation country: United States Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Mol Ther Methods Clin Dev Year: 2017 Document type: Article Affiliation country: United States Country of publication: United States