Co-Differentiation of Human Pluripotent Stem Cells-Derived Cardiomyocytes and Endothelial Cells from Cardiac Mesoderm Provides a Three-Dimensional Model of Cardiac Microtissue.

Giacomelli, Elisa; Bellin, Milena; Orlova, Valeria V; Mummery, Christine L

Giacomelli, Elisa; Bellin, Milena; Orlova, Valeria V; Mummery, Christine L.

Affiliation

Giacomelli E; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.
Bellin M; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.
Orlova VV; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.
Mummery CL; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.

Curr Protoc Hum Genet ; 95: 21.9.1-21.9.22, 2017 10 18.

Article in En | MEDLINE | ID: mdl-29044469

Subject(s)

Cell Differentiation; Endothelial Cells/cytology; Mesoderm/cytology; Myocytes, Cardiac/cytology; Pluripotent Stem Cells/cytology; Tissue Culture Techniques; Biomarkers; Cell Culture Techniques; Cell Line; Cell Separation; Cryopreservation; Endothelial Cells/metabolism; Fluorescent Antibody Technique; Humans; Myocytes, Cardiac/metabolism; Pluripotent Stem Cells/metabolism; Vascular Cell Adhesion Molecule-1/metabolism

Key words

cardiac mesoderm; cardiac microtissues; human pluripotent stem cell-derived cardiomyocytes; human pluripotent stem cell-derived endothelial cells; three-dimensional culture model

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Differentiation / Myocytes, Cardiac / Pluripotent Stem Cells / Endothelial Cells / Tissue Culture Techniques / Mesoderm Limits: Humans Language: En Journal: Curr Protoc Hum Genet Year: 2017 Document type: Article Affiliation country: Netherlands Country of publication: United States

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