Deletion of the NR4A nuclear receptor NOR1 in hematopoietic stem cells reduces inflammation but not abdominal aortic aneurysm formation.
BMC Cardiovasc Disord
; 17(1): 271, 2017 Oct 18.
Article
in En
| MEDLINE
| ID: mdl-29047330
ABSTRACT
BACKGROUND:
The NR4A3 orphan nuclear hormone receptor, NOR1, functions as a constitutively active transcription factor to regulate inflammation, proliferation, and cell survival during pathological vascular remodeling. Inflammatory processes represent key mechanisms leading to abdominal aortic aneurysm (AAA) formation. However, a role of NOR1 in AAA formation has not been investigated previously.METHODS:
Inflammatory gene expression was analyzed in bone marrow-derived macrophages isolated from NOR1-deficient mice. Low-density lipoprotein receptor-deficient (LDLr-/-) mice were irradiated and reconstituted with hematopoietic stem cells obtained from NOR1-/- or wild-type littermate mice. Animals were infused with angiotensin II and fed a diet enriched in saturated fat to induce AAA formation. Quantification of AAA formation was performed by ultrasound and ex vivo measurements.RESULTS:
Among 184 inflammatory genes that were analyzed, 36 genes were differentially regulated in LPS-treated NOR1-deficient macrophages. Albeit this difference in gene regulation, NOR1-deficiency in hematopoietic stem cells did not affect development of AAA formation in bone marrow-derived stem cell transplanted LDLr-deficient mice.CONCLUSION:
NOR1 deletion induced differential inflammatory gene transcription in macrophages but did not influence AAA formation in mice.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Receptors, Thyroid Hormone
/
Hematopoietic Stem Cells
/
Receptors, Steroid
/
Gene Deletion
/
Aortic Aneurysm, Abdominal
/
DNA-Binding Proteins
/
Nerve Tissue Proteins
Limits:
Animals
Language:
En
Journal:
BMC Cardiovasc Disord
Journal subject:
ANGIOLOGIA
/
CARDIOLOGIA
Year:
2017
Document type:
Article
Affiliation country:
United States