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Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies.
Benedikter, Birke J; Bouwman, Freek G; Vajen, Tanja; Heinzmann, Alexandra C A; Grauls, Gert; Mariman, Edwin C; Wouters, Emiel F M; Savelkoul, Paul H; Lopez-Iglesias, Carmen; Koenen, Rory R; Rohde, Gernot G U; Stassen, Frank R M.
Affiliation
  • Benedikter BJ; Department of Medical Microbiology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Center, PO box 5800, 6202AZ, Maastricht, The Netherlands.
  • Bouwman FG; Department of Respiratory Medicine, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Center, PO box 5800, 6202AZ, Maastricht, The Netherlands.
  • Vajen T; Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Center, PO box 5800, 6202AZ, Maastricht, The Netherlands.
  • Heinzmann ACA; Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, The Netherlands, PO box 616, 6200 MD, Maastricht, The Netherlands.
  • Grauls G; Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, The Netherlands, PO box 616, 6200 MD, Maastricht, The Netherlands.
  • Mariman EC; Department of Medical Microbiology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Center, PO box 5800, 6202AZ, Maastricht, The Netherlands.
  • Wouters EFM; Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Center, PO box 5800, 6202AZ, Maastricht, The Netherlands.
  • Savelkoul PH; Department of Respiratory Medicine, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Center, PO box 5800, 6202AZ, Maastricht, The Netherlands.
  • Lopez-Iglesias C; Department of Medical Microbiology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Center, PO box 5800, 6202AZ, Maastricht, The Netherlands.
  • Koenen RR; Department of Medical Microbiology & Infection Control, VU University Medical Center, Van Boechorststraat 7, 1081BT, Amsterdam, The Netherlands.
  • Rohde GGU; Microscopy Core Lab, M4I Nanoscopy division, FHML, Maastricht University, Universiteitssingel 50, G0.201, 6229 ER, Maastricht, The Netherlands.
  • Stassen FRM; Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, The Netherlands, PO box 616, 6200 MD, Maastricht, The Netherlands.
Sci Rep ; 7(1): 15297, 2017 11 10.
Article in En | MEDLINE | ID: mdl-29127410
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Chromatography, Gel / Epithelial Cells / Extracellular Vesicles Limits: Humans Language: En Journal: Sci Rep Year: 2017 Document type: Article Affiliation country: Netherlands Country of publication: United kingdom
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Chromatography, Gel / Epithelial Cells / Extracellular Vesicles Limits: Humans Language: En Journal: Sci Rep Year: 2017 Document type: Article Affiliation country: Netherlands Country of publication: United kingdom