Muprints and Whole Genome Insertion Scans: Methods for Investigating Chromosome Accessibility and DNA Dynamics using Bacteriophage Mu.

Patrick Higgins, N

Patrick Higgins, N.

Affiliation

Patrick Higgins N; Department of Biochemistry and Molecular Genetics, The University of Alabama at Birmingham, Kaul Human Genetics Building, Room 524A, 720, 20th Street South, Birmingham, AL, 35294-0024, USA. nphiggin@uab.edu.

Methods Mol Biol ; 1681: 303-314, 2018.

Article in En | MEDLINE | ID: mdl-29134604

Subject(s)

Bacteriophage mu/genetics; Chromosomes, Bacterial/genetics; DNA, Viral/genetics; Genome, Viral; Mutagenesis, Insertional/methods; DNA Transposable Elements; Electrophoresis, Agar Gel; Escherichia coli/genetics; Escherichia coli/virology; Mutagenesis, Insertional/genetics; Polymerase Chain Reaction; Temperature

Key words

Chromosome immunoprecipitation (ChIP); Chromosome immunoprecipitation sequencing (ChIP-Seq); DNA melting temperature (Tm); DNA replication; Headful DNA packaging, polymerase chain reaction (PCR); Luria Broth (LB); Plaque forming units (PFU); Transposition

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA, Viral / Mutagenesis, Insertional / Chromosomes, Bacterial / Genome, Viral / Bacteriophage mu Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2018 Document type: Article Affiliation country: United States Country of publication: United States

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