Saccharopolyspora aidingensis sp. nov., an actinomycete isolated from a salt lake.
Int J Syst Evol Microbiol
; 67(3): 687-691, 2017 Mar.
Article
in En
| MEDLINE
| ID: mdl-29144232
ABSTRACT
A novel halophilic actinomycete strain, designated TRM 46074T, was isolated from Aiding salt lake in Turpan Basin, north-west China. Strain TRM 46074T was aerobic, Gram-stain-positive and grew optimally with 12â% (w/v) NaCl. The strain was observed to produce sparse aerial mycelium with long chains of spores that were non-motile and oval or spherical in shape with a rough or smooth surface; substrate mycelium was branched and well developed. The cell wall was determined to contain meso-diaminopimelic acid; the diagnostic whole-cell sugars were galactose, arabinose and ribose. The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones were MK-9(H4), MK-10(H8) and MK-10(H4). The G+C content of the DNA was 70.9 mol%. The major fatty acids were anteiso-C17ââ0, iso-C15ââ0, iso-C16ââ0 and iso-C17ââ0. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain TRM 46074T belongs to the genus Saccharopolyspora. Strain TRM 46074T had 16S rRNA gene sequence similarity of 99.6â% with the closest described species, Saccharopolyspora ghardaiensis H53T. DNA-DNA hybridization between strain TRM 46074T and Saccharopolysporaghardaiensis H53T showed 58.6â% relatedness. On the basis of the evidence from a polyphasic study, strain TRM 46074T is considered to represent a novel species in the genus Saccharopolyspora, for which the name Saccharopolyspora aidingensis sp. nov. is proposed. The type strain is TRM 46074T (=CCTCC AA 2012014T=JCM 30185T).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phylogeny
/
Lakes
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Saccharopolyspora
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Salinity
Country/Region as subject:
Asia
Language:
En
Journal:
Int J Syst Evol Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2017
Document type:
Article