miR-596 Modulates Melanoma Growth by Regulating Cell Survival and Death.
J Invest Dermatol
; 138(4): 911-921, 2018 04.
Article
in En
| MEDLINE
| ID: mdl-29183729
ABSTRACT
Tumors grow because cancer cells lack the ability to balance cell survival and death signaling pathways. miR-596, a microRNA located at the 8p23.3 locus, has been shown by the TCGA-Assembler to be deleted in a significant number of melanoma samples. Here, we also validated the low levels of miR-596 in melanoma compared to tissue nevi, and Kaplan-Meier curve analysis revealed that low miR-596 expression was associated with worse overall survival. Moreover, we showed that miR-596 overexpression effectively inhibited MAPK/ERK signaling, cell proliferation, migration, and invasion and increased the cell apoptosis of melanoma cells. In addition, we found that miR-596 directly targets MEK1 and two apoptotic proteins, MCL1, and BCL2L1, in melanoma cells. Our findings indicated that miR-596 is an important miRNA that both negatively regulates the MAPK/ERK signaling pathway by targeting MEK1 and modulates the apoptosis pathway by targeting MCL1 and BCL2L1, suggesting that miR-596 could be a therapeutic candidate for treating melanoma, and a prognostic factor for melanoma patients.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA, Neoplasm
/
Gene Expression Regulation, Neoplastic
/
MicroRNAs
/
Melanoma
Type of study:
Prognostic_studies
Limits:
Female
/
Humans
Language:
En
Journal:
J Invest Dermatol
Year:
2018
Document type:
Article
Affiliation country:
Taiwan