Reduced Lipocalin 2 Expression Contributes to Vincristine Resistance in Human Colon Cancer Cells.
Recent Pat Anticancer Drug Discov
; 13(2): 248-254, 2018.
Article
in En
| MEDLINE
| ID: mdl-29268690
ABSTRACT
BACKGROUND:
Vincristine (VCR) resistance can lead to cancer chemotherapy failure. Although changes in gene expression are responsible for drug resistance, the specific identities and roles of these genes remain unclear.OBJECTIVE:
In this study, we aimed to identify differentially expressed genes and mechanisms of VCR resistance in colorectal cancer (CRC) cells.METHODS:
A VCR-resistant CRC cell line (HCT-8/VCR) was established, and differentially expressed proteins between HCT-8 and HCT-8/VCR cells were screened using a human cytokine array; the results were confirmed by reverse transcription polymerase chain reaction and Western blotting. Furthermore, differentially expressed proteins were downregulated using siRNA, and cell proliferation and apoptosis were assessed by Cell Counting Kit-8 assay and flow cytometry, respectively.RESULTS:
Compared with HCT-8 CRC cells, HCT-8/VCR cells showed downregulation of lipocalin 2 (LCN2). We found that siRNA-mediated downregulation of LCN2 in HCT-8 cells significantly increased VCR resistance. Furthermore, when we downregulated LCN2, we observed significant decreases in apoptosis, but no significant effect on cell cycle.CONCLUSION:
Overall, these results demonstrate that LCN2 plays an important role in VCR resistance and is a potential therapeutic target for this disease.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Vincristine
/
Gene Expression Regulation, Neoplastic
/
Colonic Neoplasms
/
Drug Resistance, Neoplasm
/
Lipocalin-2
/
Antineoplastic Agents, Phytogenic
Limits:
Humans
Language:
En
Journal:
Recent Pat Anticancer Drug Discov
Journal subject:
NEOPLASIAS
/
TERAPIA POR MEDICAMENTOS
Year:
2018
Document type:
Article
Affiliation country:
China
Publication country:
AE
/
EMIRADOS ÁRABES UNIDOS
/
EMIRATOS ARABES UNIDOS
/
UNITED ARAB EMIRATES