[Bile-resistant Gram-negative bacteria effect of different kinds of root decoction pieces].
Zhongguo Zhong Yao Za Zhi
; 42(21): 4135-4141, 2017 Nov.
Article
in Zh
| MEDLINE
| ID: mdl-29271151
ABSTRACT
To investigate the microbial contamination in Chinese herbal decoction pieces with different functional types by studying the total aerobic microbial count (TAMC), and total yeast and mould count (TYMC) in 40 samples of 8 types of root decoction pieces; further evaluate the contamination load of bile-resistant Gram-negative bacteria, and identify the Gram-negative bacteria by using biochemical identification system for Gram-negative bacteria. Our results showed that the TAMC value was more than 1 000 CFUâ¢g⻹ in 85% (34/40) samples, and was more than 100 CFUâ¢g⻹ in 30% (12/40) samples; the contamination of bile-resistant Gram-negative bacteria was detected in 45% (18/40) of the samples. The bile-resistant Gram-negative bacteria load of seven batches of samples was N>1 000 MPNâ¢g⻹. Sixteen bacterium strains including Serratia plymouthensis, Cedecea neteri, Escherichia vulneris, Klebsiella oxytoca, Enterobacter amnigenus, E. cloacae, E. sakazakii, Proteus penneri and E. gergoviae were obtained and identified. E. cloacae was the predominant bacterium that was isolated from Salviae Miltiorrhizae Radix et Rhizoma, while E. amnigenus, Yersinia pseudotuberculosis was the typical bacterium of Ophiopogonis Radix and Codonopsis Radix, respectively. All these suggested that the contamination of bile-resistant Gram-negative bacteria was severe for the root decoction pieces in Wuhan city. Microbial species have certain selection specificity for medicinal ingredients, so the type and limit of control bacteria for detection should be formulated according to the pollution type and quantity of bile-resistant Gram-negative bacteria.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bile
/
Drugs, Chinese Herbal
/
Plant Roots
/
Gram-Negative Bacteria
/
Anti-Bacterial Agents
Language:
Zh
Journal:
Zhongguo Zhong Yao Za Zhi
Journal subject:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Year:
2017
Document type:
Article
Affiliation country:
China