In vitro effects of virgin microplastics on fish head-kidney leucocyte activities.
Environ Pollut
; 235: 30-38, 2018 Apr.
Article
in En
| MEDLINE
| ID: mdl-29274535
ABSTRACT
Microplastics are well-documented pollutants in the marine environment that result from production or fragmentation of larger plastic items. The knowledge about the direct effects of microplastics on immunity, including fish, is still very limited. We investigated the in vitro effects of microplastics [polyvinylchloride (PVC) and polyethylene (PE)] on gilthead seabream (Sparus aurata) and European sea bass (Dicentrarchus labrax) head-kidney leucocytes (HKLs). After 1 and 24â¯h of exposure of HKLs with 0 (control), 1, 10 and 100â¯mgâ¯mL-1 MPs in a rotatory system, cell viability, innate immune parameters (phagocytic, respiratory burst and peroxidase activities) and the expression of genes related to inflammation (il1b), oxidative stress (nrf2, prdx3), metabolism of xenobiotics (cyp1a1, mta) and cell apoptosis (casp3) were studied. Microplastics failed to affect the cell viability of HKLs. In addition, they provoke very few significant effects on the main cellular innate immune activities, as decrease on phagocytosis or increase in the respiratory burst of HKLs with the highest dose of microplastics tested. Furthermore, microplastics failed to affect the expression of the selected genes on sea bass or seabream, except the nrf2 which was up-regulated in seabream HKLs incubated with the highest doses. Present results seem to suggest that continue exposure of fish to PVC or PE microplastics could impair fish immune parameters probably due to the oxidative stress produced in the fish leucocytes.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polyvinyl Chloride
/
Water Pollutants
/
Bass
/
Polyethylene
/
Sea Bream
/
Head Kidney
/
Leukocytes
Limits:
Animals
Language:
En
Journal:
Environ Pollut
Journal subject:
SAUDE AMBIENTAL
Year:
2018
Document type:
Article
Affiliation country:
Spain