RFX1 and RFX3 Transcription Factors Interact with the D Sequence of Adeno-Associated Virus Inverted Terminal Repeat and Regulate AAV Transduction.
Sci Rep
; 8(1): 210, 2018 01 09.
Article
in En
| MEDLINE
| ID: mdl-29317724
ABSTRACT
Adeno-associated virus (AAV) transduction efficiency depends on the way in which cellular proteins process viral genomes in the nucleus. In this study, we have investigated the binding of nuclear proteins to the double stranded D (dsD) sequence of the AAV inverted terminal repeat (ITRs) by electromobility shift assay. We present here several lines of evidence that transcription factors belonging to the RFX protein family bind specifically and selectively to AAV2 and AAV1 dsD sequences. Using supershift experiments, we characterize complexes containing RFX1 homodimers and RFX1/RFX3 heterodimers. Following transduction of HEK-293 cells, the AAV genome can be pulled-down by RFX1 and RFX3 antibodies. Moreover, our data suggest that RFX proteins which interact with transcriptional enhancers of several mammalian DNA viruses, can act as regulators of AAV mediated transgene expression.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transduction, Genetic
/
Dependovirus
/
Regulatory Factor X Transcription Factors
/
Regulatory Factor X1
Type of study:
Risk_factors_studies
Limits:
Humans
Language:
En
Journal:
Sci Rep
Year:
2018
Document type:
Article
Affiliation country:
France