The effect of thiamine and its metabolites on peripheral neuropathic pain induced by cisplatin in rats.
Exp Anim
; 67(2): 259-269, 2018 May 10.
Article
in En
| MEDLINE
| ID: mdl-29332858
ABSTRACT
Thiamine pyrophosphate (TPP) is the active metabolite of thiamine. This study aimed to investigate the effects of thiamine and TPP on cisplatin-induced peripheral neuropathic pain (PNP). Male albino Wistar type Rattus norvegicus were divided into six groups (n=6) that received 2 mg/kg cisplatin (CIS), 25 mg/kg thiamine (TM), 2 mg/kg cisplatin+25 mg/kg thiamine (CTM), 25 mg/kg TPP (TPP), 2 mg/kg cisplatin+25 mg/kg TPP (CTPP), or distilled water (healthy group; HG) for 8 days intraperitoneally. Analgesic effect was measured with a Basile Algesimeter. IL-1ß, malondialdehyde (MDA), total glutathione (tGSH), thiamine, and TPP were determined in blood samples. Histopathological examinations were performed on removed sciatic nerves. The percent analgesic effects of the CTM and CTPP groups were calculated to be 21.3% and 82.9%, respectively. Increased production of IL-1ß and MDA by cisplatin was inhibited by TPP, while it was not inhibited by thiamine. Conversion of thiamine to TPP significantly decreased in the CIS group. Histopathological and biochemical investigations demonstrated that hyperalgesia and sciatic nerve damage developed in the CIS and CTM groups with low TPP levels. These results indicate that cisplatin inhibits the formation of TPP from thiamine, leading to severe PNP. This finding suggests that TPP may be more beneficial than thiamine for the treatment of cisplatin-induced PNP.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Thiamine
/
Thiamine Pyrophosphate
/
Cisplatin
/
Peripheral Nervous System Diseases
/
Analgesics
/
Neuralgia
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Exp Anim
Journal subject:
MEDICINA VETERINARIA
Year:
2018
Document type:
Article
Affiliation country:
Turkey