Highly parallel direct RNA sequencing on an array of nanopores.
Nat Methods
; 15(3): 201-206, 2018 03.
Article
in En
| MEDLINE
| ID: mdl-29334379
ABSTRACT
Sequencing the RNA in a biological sample can unlock a wealth of information, including the identity of bacteria and viruses, the nuances of alternative splicing or the transcriptional state of organisms. However, current methods have limitations due to short read lengths and reverse transcription or amplification biases. Here we demonstrate nanopore direct RNA-seq, a highly parallel, real-time, single-molecule method that circumvents reverse transcription or amplification steps. This method yields full-length, strand-specific RNA sequences and enables the direct detection of nucleotide analogs in RNA.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Saccharomyces cerevisiae
/
RNA, Fungal
/
Sequence Analysis, RNA
/
Saccharomyces cerevisiae Proteins
/
High-Throughput Nucleotide Sequencing
/
Nanopores
Language:
En
Journal:
Nat Methods
Journal subject:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Year:
2018
Document type:
Article
Affiliation country:
United kingdom