Pyridone Luciferins and Mutant Luciferases for Bioluminescence Imaging.
Chembiochem
; 19(5): 470-477, 2018 03 02.
Article
in En
| MEDLINE
| ID: mdl-29384255
ABSTRACT
New applications for bioluminescence imaging require an expanded set of luciferase enzymes and luciferin substrates. Here, we report two novel luciferins for use in vitro and in cells. These molecules comprise regioisomeric pyridone cores that can be accessed from a common synthetic route. The analogues exhibited unique emission spectra with firefly luciferase, although photon intensities remained weak. Enhanced light outputs were achieved by using mutant luciferase enzymes. One of the luciferin-luciferase pairs produced light on par with native probes in live cells. The pyridone analogues and complementary luciferases add to a growing set of designer probes for bioluminescence imaging.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pyridones
/
Firefly Luciferin
/
Luciferases, Firefly
/
Luminescent Agents
/
Optical Imaging
/
Mutation
Limits:
Animals
/
Humans
Language:
En
Journal:
Chembiochem
Journal subject:
BIOQUIMICA
Year:
2018
Document type:
Article
Affiliation country:
United States