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Establishment and characterization of a human embryonic stem cell line, NERCe002-A-3, with inducible 14-3-3ζ overexpression.
He, Jingjing; Duan, Xingxiang; Li, Wen; Peng, Yingying; Yu, Juan; Hu, Liang; Zeng, Sicong; Wang, Yang; Lu, Guangxiu; Lin, Ge; Sun, Yi.
Affiliation
  • He J; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
  • Duan X; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
  • Li W; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China.
  • Peng Y; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
  • Yu J; National Engineering and Research Center of Human Stem Cells, Changsha, China.
  • Hu L; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, Chi
  • Zeng S; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China.
  • Wang Y; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
  • Lu G; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China.
  • Lin G; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, Chi
  • Sun Y; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, Chi
Stem Cell Res ; 28: 11-15, 2018 04.
Article in En | MEDLINE | ID: mdl-29414411
ABSTRACT
NERCe002-A-3 cells were generated from the normal human embryonic stem cell line NERCe002-A. NERCe002-A-3 cells overexpressed 14-3-3ζ after exposure to doxycycline. 14-3-3ζ protein have the ability to bind a multitude of functionally diverse signalling proteins. The NERCe002-A-3 cell line is considered a model for functional studies of the 14-3-3ζ protein in hESC self-renewal and cell differentiation. Doxycycline-treated NERCe002-A-3 cells showed a>27-fold increase in relative expression of 14-3-3ζ as compared with un-induced cells. Characterization assays proved that NERCe002-A-3 cells express typical markers of pluripotency and have the ability to form the three germ layers in vivo.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Culture Techniques / 14-3-3 Proteins / Human Embryonic Stem Cells Type of study: Prognostic_studies Limits: Animals / Humans / Male Language: En Journal: Stem Cell Res Year: 2018 Document type: Article Affiliation country: China
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Culture Techniques / 14-3-3 Proteins / Human Embryonic Stem Cells Type of study: Prognostic_studies Limits: Animals / Humans / Male Language: En Journal: Stem Cell Res Year: 2018 Document type: Article Affiliation country: China