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Generation of a human embryonic stem cell line expressing tetrameric Zoanthus sp. green fluorescent protein: NERCe002-A-1.
Duan, Xingxiang; Xie, Menghan; Peng, Yingying; Hu, Liang; Yu, Juan; Zeng, Sicong; Wang, Yang; Lu, Guangxiu; Lin, Ge; Sun, Yi.
Affiliation
  • Duan X; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
  • Xie M; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
  • Peng Y; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
  • Hu L; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, Chin
  • Yu J; National Engineering and Research Center of Human Stem Cell, Changsha, China.
  • Zeng S; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cell, Changsha, China.
  • Wang Y; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
  • Lu G; National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China.
  • Lin G; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, Chin
  • Sun Y; Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, Chin
Stem Cell Res ; 28: 6-10, 2018 04.
Article in En | MEDLINE | ID: mdl-29414419
ABSTRACT
The human embryonic stem cell (hESC) line NERCe002-A-1 was generated through lentiviral transduction of the original NERCe002-A-1 hESC line with Zoanthus sp. green fluorescent protein (ZsGreen). Cells that expressed ZsGreen showed a >8.6-fold increase in fluorescence intensity compared with that of cells that expressed enhanced green fluorescent protein. The fluorescent hESC line can aid in identification of biological characteristics in vitro and in vivo by tracking cell growth, migration, and differentiation. Characteristic tests confirmed that the NERCe002-A-1 cell line expressed typical markers of pluripotency and had the capability to form the three germ layers in vivo.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Culture Techniques / Anthozoa / Green Fluorescent Proteins / Protein Multimerization / Human Embryonic Stem Cells Type of study: Prognostic_studies Limits: Animals / Humans / Male Language: En Journal: Stem Cell Res Year: 2018 Document type: Article Affiliation country: China
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Culture Techniques / Anthozoa / Green Fluorescent Proteins / Protein Multimerization / Human Embryonic Stem Cells Type of study: Prognostic_studies Limits: Animals / Humans / Male Language: En Journal: Stem Cell Res Year: 2018 Document type: Article Affiliation country: China