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Different expression profiles of the lysyl oxidases and matrix metalloproteinases in human ACL fibroblasts after co-culture with synovial cells.
Wang, Chunli; Xu, Chunming; Chen, Rongfu; Yang, Li; Sung, Kl Paul.
Affiliation
  • Wang C; a "111" project Laboratory of Biomechanics and Tissue Repair, Bioengineering College , Chongqing University , Chongqing , China.
  • Xu C; a "111" project Laboratory of Biomechanics and Tissue Repair, Bioengineering College , Chongqing University , Chongqing , China.
  • Chen R; b Department of Orthopedics , People's hospital of changshou Chongqing , Chongqing , China.
  • Yang L; a "111" project Laboratory of Biomechanics and Tissue Repair, Bioengineering College , Chongqing University , Chongqing , China.
  • Sung KP; a "111" project Laboratory of Biomechanics and Tissue Repair, Bioengineering College , Chongqing University , Chongqing , China.
Connect Tissue Res ; 59(4): 369-380, 2018 07.
Article in En | MEDLINE | ID: mdl-29431515
Purposes The anterior cruciate ligament (ACL) has poor functional healing response. The synovial tissue surrounding ACL ligament might be a major regulator of the microenvironment in the joint cavity after ACL injury, thus affecting the repair process. Using transwell co-culture, this study explored the direct influence of human synovial cells (HSCs) on ACL fibroblasts (ACLfs) by characterizing the differential expression of the lysyl oxidase family (LOXs) and matrix metalloproteinases (MMP-1, -2, -3), which facilitate extracellular matrix (ECM) repair and degradation, respectively. Methods The mRNA expression levels of LOXs and MMP-1, -2, -3 were analyzed by semi-quantitative PCR and quantitative real-time PCR. The protein expression levels of LOXs and MMP-1, -2, -3 were detected by western blot. Results We found that co-culture resulted in an increase in the mRNAs of LOXs in normal ACLfs and differentially regulated the expression of MMPs. Then we applied 12% mechanical stretch on ACLfs to induce injury and found the mRNA expression levels of LOXs in injured ACLfs were decreased in the co-culture group relative to the mono-culture group. Conversely, the mRNA expression levels of MMPs in injured ACLfs were promoted in the co-culture group compared with the mono-culture group. At translational level, we found that LOXs were lower while MMPs were highly expressed in the co-culture group compared to the mono-culture group. Conclusions The co-culture of ACLfs and HSCs, which mimicked the cell-to-cell contact in a micro-environment, could contribute to protein modulators for wound healing, inferring the potential reason for the poor self-healing of injured ACL.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Synovial Membrane / Anterior Cruciate Ligament / Matrix Metalloproteinases / Gene Expression Profiling / Fibroblasts / Protein-Lysine 6-Oxidase Limits: Adult / Female / Humans / Male / Middle aged Language: En Journal: Connect Tissue Res Year: 2018 Document type: Article Affiliation country: China Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Synovial Membrane / Anterior Cruciate Ligament / Matrix Metalloproteinases / Gene Expression Profiling / Fibroblasts / Protein-Lysine 6-Oxidase Limits: Adult / Female / Humans / Male / Middle aged Language: En Journal: Connect Tissue Res Year: 2018 Document type: Article Affiliation country: China Country of publication: United kingdom