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Miniaturizing EM Sample Preparation: Opportunities, Challenges, and "Visual Proteomics".
Arnold, Stefan A; Müller, Shirley A; Schmidli, Claudio; Syntychaki, Anastasia; Rima, Luca; Chami, Mohamed; Stahlberg, Henning; Goldie, Kenneth N; Braun, Thomas.
Affiliation
  • Arnold SA; Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel, Switzerland.
  • Müller SA; Swiss Nanoscience Institute, University of Basel, Basel, Switzerland.
  • Schmidli C; Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel, Switzerland.
  • Syntychaki A; Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel, Switzerland.
  • Rima L; Swiss Nanoscience Institute, University of Basel, Basel, Switzerland.
  • Chami M; Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel, Switzerland.
  • Stahlberg H; Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel, Switzerland.
  • Goldie KN; BioEM Lab, Biozentrum, University of Basel, Basel, Switzerland.
  • Braun T; Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel, Switzerland.
Proteomics ; 18(5-6): e1700176, 2018 03.
Article in En | MEDLINE | ID: mdl-29441686
ABSTRACT
This review compares and discusses conventional versus miniaturized specimen preparation methods for transmission electron microscopy (TEM). The progress brought by direct electron detector cameras, software developments and automation have transformed transmission cryo-electron microscopy (cryo-EM) and made it an invaluable high-resolution structural analysis tool. In contrast, EM specimen preparation has seen very little progress in the last decades and is now one of the main bottlenecks in cryo-EM. Here, we discuss the challenges faced by specimen preparation for single particle EM, highlight current developments, and show the opportunities resulting from the advanced miniaturized and microfluidic sample grid preparation methods described, such as visual proteomics and time-resolved cryo-EM studies.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Proteins / Cryoelectron Microscopy / Imaging, Three-Dimensional / Proteomics / Microscopy, Electron, Transmission Limits: Humans Language: En Journal: Proteomics Journal subject: BIOQUIMICA Year: 2018 Document type: Article Affiliation country: Switzerland
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Proteins / Cryoelectron Microscopy / Imaging, Three-Dimensional / Proteomics / Microscopy, Electron, Transmission Limits: Humans Language: En Journal: Proteomics Journal subject: BIOQUIMICA Year: 2018 Document type: Article Affiliation country: Switzerland