Identification of branched-chain amino acid aminotransferases active towards (R)-(+)-1-phenylethylamine among PLP fold type IV transaminases.
J Biotechnol
; 271: 26-28, 2018 Apr 10.
Article
in En
| MEDLINE
| ID: mdl-29453991
ABSTRACT
New class IV transaminases with activity towards L-Leu, which is typical of branched-chain amino acid aminotransferases (BCAT), and with activity towards (R)-(+)-1-phenylethylamine ((R)-PEA), which is typical of (R)-selective (R)-aminepyruvate transaminases, were identified by bioinformatics analysis, obtained in recombinant form, and analyzed. The values of catalytic activities in the reaction with L-Leu and (R)-PEA are comparable to those measured for characteristic transaminases with the corresponding specificity. Earlier, (R)-selective class IV transaminases were found to be active, apart from (R)-PEA, only with some other (R)-primary amines and D-amino acids. Sequences encoding new transaminases with mixed type of activity were found by searching for changes in the conserved motifs of sequences of BCAT by different bioinformatics tools.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phenethylamines
/
Bacteria
/
Computational Biology
/
Transaminases
Type of study:
Diagnostic_studies
/
Prognostic_studies
Language:
En
Journal:
J Biotechnol
Journal subject:
BIOTECNOLOGIA
Year:
2018
Document type:
Article