True gene-targeting events by CRISPR/Cas-induced DSB repair of the PPO locus with an ectopically integrated repair template.

de Pater, Sylvia; Klemann, Bart J P M; Hooykaas, Paul J J

de Pater, Sylvia; Klemann, Bart J P M; Hooykaas, Paul J J.

Affiliation

de Pater S; Department of Molecular and Developmental Genetics, Institute of Biology, Leiden University, Leiden, 2333 BE, The Netherlands. b.s.de.pater@biology.leidenuniv.nl.
Klemann BJPM; Department of Molecular and Developmental Genetics, Institute of Biology, Leiden University, Leiden, 2333 BE, The Netherlands.
Hooykaas PJJ; Dümmen Orange, De Lier, 2678 PS, The Netherlands.

Sci Rep ; 8(1): 3338, 2018 02 20.

Article in En | MEDLINE | ID: mdl-29463822

Subject(s)

Arabidopsis/genetics; CRISPR-Cas Systems; DNA Repair; DNA, Plant/genetics; Gene Targeting/methods; Protoporphyrinogen Oxidase/genetics; Agrobacterium tumefaciens/genetics; Arabidopsis/enzymology; Arabidopsis Proteins/antagonists & inhibitors; Arabidopsis Proteins/genetics; DNA Breaks, Double-Stranded; Geminiviridae/genetics; Genome, Plant; Homologous Recombination; Protoporphyrinogen Oxidase/antagonists & inhibitors

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Arabidopsis / Gene Targeting / DNA, Plant / DNA Repair / Protoporphyrinogen Oxidase / CRISPR-Cas Systems Language: En Journal: Sci Rep Year: 2018 Document type: Article Affiliation country: Netherlands Country of publication: United kingdom

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